New Study Shows Similar Bioavailability of Sulforaphane from Commercially Available Glucoraphanin Supplements and Broccoli Sprouts

Findings Validate the Use of High-Quality Glucoraphanin Supplements to Help Support the Body’s Detoxification Pathways and Eliminate Toxins

BALTIMORE, MARYLAND – November 11, 2015 – Decades of research supports the efficacy of sulforaphane as a way to eliminate toxins from the body. A new cross-over clinical study conducted at Johns Hopkins University (JHU) School of Medicine shows glucoraphanin (sulforaphane’s dietary precursor) from broccoli seeds and broccoli sprouts are equally bioavailable, validating the use of high-quality glucoraphanin supplements from broccoli seeds to help support the body’s detoxification pathways. The paper titled, “Sulforaphane Bioavailability from Glucoraphanin-Rich Broccoli: Control by Active Endogenous Myrosinase,” was recently published in the open access journal Public Library of Science (PLoS ONE).

“This was the first study in which we used a commercially available glucoraphanin-rich product made from broccoli seeds. The study demonstrated that these specific glucoraphanin supplements are equally bioavailable to the glucoraphanin-rich broccoli sprout extract we have used in previous clinical studies,” said lead author Jed Fahey, Sc.D., Johns Hopkins University School of Medicine. “From a public health perspective, this study will pave the way for future research on the efficacy of over-the-counter glucoraphanin supplements.”

Clinical Cross-Over Trial Design and Outcomes
The Johns Hopkins University (JHU) School of Medicine researchers recruited a group of 20 participants with an average age of 51 years. The researchers gave participants the following glucoraphanin preparations:

  • JHU-made broccoli sprout extract containing 30 mg or 100 mg of glucoraphanin
  • OncoPLEX™ supplements containing 30 mg or 100 mg of glucoraphanin

Throughout the intervention phase, the trial and during urine analyses, the researchers were blinded to the source of glucoraphanin. Each participant received one dose of each preparation, and doses were separated by one week. This method helped to control for the variability in the participant’s metabolism and digestive microflora, which can influence the activity of glucoraphanin. The study design enhanced the power of the test because each subject was their own control, and each of the 17 subjects who completed the study consumed each of the four test preparations in random order.

In this study, the researchers measured the excretion of sulforaphane metabolites in the participants’ urine, comparing the JHU-made broccoli sprout extract to the OncoPLEX supplements from broccoli seeds. The researchers found that these two sources were equally bioavailable. For the 30 mg glucoraphanin doses, the average conversions were 12.8 percent for the JHU broccoli sprout preparation and 11.2 percent for the OncoPLEX broccoli seed supplement preparation. For the 100 mg dose of JHU-preparation and OncoPLEX supplement, the average conversion rates for the two preparations were 8.3 percent and 9.7 percent, respectively. Statistically, results from each preparation were equivalent.

“The recent trial at JHU Medical School demonstrates that glucoraphanin from broccoli seeds is equally as bioavailable as glucoraphanin from the broccoli sprouts that have been used by Johns Hopkins researchers and others in large-scale human trials throughout the world,” commented Tony Talalay, CEO of Brassica Protection Products LLC. “While supplements are not intended to substitute for a balanced diet with nutrient-packed vegetables such as broccoli, consumers now have the assurance of knowing that high-quality glucoraphanin supplements from broccoli seeds can help to support the body’s own natural detoxification processes.”

The independent investigator-initiated clinical trial was conducted at Johns Hopkins University School of Medicine and partially supported by an unrestricted grant from the Lewis B. and Dorothy Cullman Foundation. Brassica Protection Products, makers of truebroc™ glucoraphanin, provided its truebroc glucoraphanin powder to Xymogen that in turn encapsulated the sprout extract and OncoPLEX™ preparations in identical capsules for use in the study.

truebroc™ Glucoraphanin

Nearly 40 percent of Americans wish they could get the nutritional benefits of broccoli without actually having to eat it, according to the 2015 Brassica survey. truebroc™ branded glucoraphanin is the most concentrated source of glucoraphanin from broccoli currently on the market and can be found in functional beverages and dietary supplements, including OncoPLEX used in the PLoS ONE study. Look for the truebroc logo to be sure you are getting a high-quality source of glucoraphanin from broccoli.

Brassica Protection Products, LLC

Brassica Protection Products, founded in 1997, is an innovator in the research and development of nutritional ingredients derived from cruciferous vegetables, specifically broccoli. Brassica extracts its glucoraphanin from broccoli seeds and sells its truebroc™ glucoraphanin for use in supplements, functional foods and beverages. Brassica also sells Brassica® Tea, a line of gourmet green and black teas containing truebroc glucoraphanin. For more information about Brassica Protection Products, visit

For more information:

Brassica Protection Products

Sarah Sullivan

phone: 410.732.1200 x105



Andrea D. Martin (on behalf of Brassica)

Andrea D. Martin Consulting, LLC

phone: 443.927.6183




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